Transient gene expression in untransformed lymphocytes
Copyright policy )Transient gene expression in untransformed lymphocytes
AbstractConditions for transfection of normal, freshly isolated and mitogen‐stimulated spleen cells were established. A sharp optimum of plasmid at 10 μg per 1.5 × 107 transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly isolated lymphocytes in vitro.
- Uppsala University Sweden
Chloramphenicol O-Acetyltransferase, Male, Lymphoma, Immunoglobulins, Transfection, Mice, Inbred C57BL, Mice, Enhancer Elements, Genetic, Acetyltransferases, Organ Specificity, Mice, Inbred CBA, Tumor Cells, Cultured, Animals, Female, Lymphocytes, Promoter Regions, Genetic
Chloramphenicol O-Acetyltransferase, Male, Lymphoma, Immunoglobulins, Transfection, Mice, Inbred C57BL, Mice, Enhancer Elements, Genetic, Acetyltransferases, Organ Specificity, Mice, Inbred CBA, Tumor Cells, Cultured, Animals, Female, Lymphocytes, Promoter Regions, Genetic
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