SUMMARYThe use of cilia from microorganisms requires design of the channel and the fabrication process. We developed a method of injecting Vorticella into a microchamber and exploited the properties of Vorticella as a micromixer of two solutions. We characterized a flow field generated by the ciliary motion of Vorticella. We measured the stability and mixing performance of Vorticella by particle image velocimetry (PIV). Our PIV measurements showed that a single Vorticella mixes the half‐area of a microchamber (200 × 400 µm). We conclude that the flow field of a single cell in a chamber is more stable than that of multiple cells. © 2013 Wiley Periodicals, Inc. Electron Comm Jpn, 96(10): 43–49, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/ecj.11468