A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live‐imaging
Copyright policy )A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live‐imaging
AbstractLive‐imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R‐RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live‐imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R‐RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations. genesis 49:570–578, 2011. © 2011 Wiley‐Liss, Inc.
- Kyoto University Japan
- Baylor College of Medicine United States
- National Institutes of Natural Sciences Japan
- The University of Texas MD Anderson Cancer Center United States
- RIKEN Center for Biosystems Dynamics Research Japan
Cell Nucleus, Male, Genotype, Staining and Labeling, Cell Membrane, Embryonic Development, Gene Expression Regulation, Developmental, Mice, Transgenic, Time-Lapse Imaging, Molecular Imaging, Mice, Genetic Loci, Organ Specificity, Gene Order, Gene Targeting, Animals, Female
Cell Nucleus, Male, Genotype, Staining and Labeling, Cell Membrane, Embryonic Development, Gene Expression Regulation, Developmental, Mice, Transgenic, Time-Lapse Imaging, Molecular Imaging, Mice, Genetic Loci, Organ Specificity, Gene Order, Gene Targeting, Animals, Female
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