Stella‐Cre mice are highly efficient Cre deleters
Copyright policy )Stella‐Cre mice are highly efficient Cre deleters
AbstractCre‐loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella‐Cre, where Cre expression cassette was targeted to the 3′ UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre‐loxP recombination efficiency in Stella‐Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre‐loxP recombination efficiency in the embryos and in the germline. Thus, Stella‐Cre mice serve as a very efficient deleter line. genesis 49:689–695, 2011. © 2011 Wiley‐Liss, Inc.
- University of Cambridge United Kingdom
- Wellcome Sanger Institute United Kingdom
- University of Hong Kong China (People's Republic of)
- Huazhong Agricultural University China (People's Republic of)
- University of Hong Kong (香港大學) China (People's Republic of)
Male, Knock-in, Mice, 129 Strain, Chromosomal Proteins, Non-Histone, Gene Expression, Mice, Transgenic, Mice, Technology Reports, Animals, Bacteriophage P1, 3' Untranslated Regions, Integrases, Nuclear Proteins, Proteins, Cre-loxP recombination, Janus Kinase 2, Embryo, Mammalian, Flow Cytometry, Stella, Blastocyst, Germ Cells, Attachment Sites, Microbiological, Female, Nucleophosmin
Male, Knock-in, Mice, 129 Strain, Chromosomal Proteins, Non-Histone, Gene Expression, Mice, Transgenic, Mice, Technology Reports, Animals, Bacteriophage P1, 3' Untranslated Regions, Integrases, Nuclear Proteins, Proteins, Cre-loxP recombination, Janus Kinase 2, Embryo, Mammalian, Flow Cytometry, Stella, Blastocyst, Germ Cells, Attachment Sites, Microbiological, Female, Nucleophosmin
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