RNA Extraction and Preparation in Rice (Oryza sativa)

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Mar 2016 English Publisher:WileyJournal:Current Protocols in Plant Biology, volume 1, pages 411-418 (issn: 2379-8068, eissn: 2379-8068,

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Authors: Cui-Cui, Yin; Biao, Ma; Wei, Wang; Qing, Xiong; He, Zhao; Shou-Yi, Chen; Jin-Song, Zhang;

doi: 10.1002/cppb.20023

pmid: 30775862

RNA Extraction and Preparation in Rice (Oryza sativa)

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Abstract

AbstractThere has been a surge of interest in the molecular biology of RNA. We describe a simple, fast, and convenient protocol for isolating total RNA from rice (Oryza sativa) seedlings and young tissues/organs. RNA obtained using this method can be used for reverse transcription‐polymerase chain reaction (RT‐PCR), quantitative RT‐PCR (qRT‐PCR), northern blot analysis, RNA‐seq, microarray analysis, cDNA library construction, etc. Technical parameters critical to the success of the extraction are presented. © 2016 by John Wiley & Sons, Inc.

Related Organizations

Institute of Genetics and Developmental Biology
China (People's Republic of)
Chinese Academy of Sciences
China (People's Republic of)

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