The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus

descriptionPublicationkeyboard_double_arrow_right Article 24 Aug 2020 English Publisher:WileyJournal:Current Protocols in Microbiology, volume 58 (issn: 1934-8525, eissn: 1934-8533,

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Authors: Alaa A, Abdelmageed; Maureen C, Ferran;

doi: 10.1002/cpmc.110

pmid: 32833351

pmc: PMC7449588

The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus

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Abstract

AbstractVesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative‐sense single‐stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Generation, purification, and storage of vesicular stomatitis virus stocksBasic Protocol 2: Quantification of vesicular stomatitis virus by plaque assaySupport Protocol: Propagation of Vero cells

Related Organizations

Rochester Institute of Technology
United States

Keywords

Virus Cultivation, Preservation, Biological, Viral Plaque Assay, Vesicular stomatitis Indiana virus, Cell Line, Specimen Handling, Chlorocebus aethiops, Animals, Humans, Vesicular Stomatitis, Vero Cells

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