Cytomegalovirus Infection: Mouse Model

descriptionPublicationkeyboard_double_arrow_right Article 25 Jul 2018 Germany, Croatia English Publisher:WileyJournal:Current Protocols in Immunology, volume 122 (issn: 1934-3671, eissn: 1934-368X,

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Authors: Brizić, Ilija; Lisnić, Berislav; Brune, Wolfram; Hengel, Hartmut; Jonjić, Stipan;

doi: 10.1002/cpim.51

pmid: 30044539

pmc: PMC6347558

Cytomegalovirus Infection: Mouse Model

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Abstract

AbstractThis unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay. © 2018 by John Wiley & Sons, Inc.

Countries

Germany, Croatia

Related Organizations

Leibniz Association
Germany
University of Freiburg
Germany
University of Rijeka
Croatia
SVEUCILISTE U ZAGREBU FAKULTET ELEKTROTEHNIKE I RACUNARSTVA
Croatia
Heinrich-Pette-Institute
Germany

Keywords

salivary gland/virology, mice, disease models, viral plaque assay/methods, 610, herpesviridae infections/virology, viral load, mouse cytomegalovirus/isolation & purification, animal, cytomegalovirus infections/virology, mice ; cytomegalovirus infections/virology ; disease models ; animal ; virus replication ; viral plaque assay/methods ; viral load ; salivary gland/virology ; mouse cytomegalovirus/isolation & purification ; herpesviridae infections/virology ; fibroblasts/virology ; MCMV, virus replication, fibroblasts/virology, MCMV

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