A Force‐Spectroscopy‐Based Single‐Molecule Metal‐Binding Assay

descriptionPublicationkeyboard_double_arrow_right Article 29 Jun 2009 English Publisher:WileyJournal:ChemPhysChem, volume 10, pages 1,450-1,454 (issn: 1439-4235, eissn: 1439-7641,

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Authors: Yi, Cao; Kai Shih, Er; Rakesh, Parhar; Hongbin, Li;

doi: 10.1002/cphc.200900333

pmid: 19514035

A Force‐Spectroscopy‐Based Single‐Molecule Metal‐Binding Assay

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Abstract

AbstractQuantifying metal‐binding by force: A quantitative single‐molecule force‐spectroscopy‐based assay is developed to measure the binding affinity of metal ions to proteins. The method uses the unfolding force of a protein as a direct probe to distinguish the apo and metal‐ion‐bound forms of that protein and quantify the partitioning between the two forms (see figure).magnified image

Related Organizations

University of British Columbia
Canada

Keywords

Kinetics, Metals, Proteins, Thermodynamics, Spectrophotometry, Ultraviolet, Microscopy, Atomic Force, Recombinant Proteins, Protein Binding

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