Immunoprecipitation
Copyright policy )Immunoprecipitation
AbstractSelective immunoprecipitation of proteins is a useful tool for characterizing proteins and protein‐protein interactions. Clear step‐by‐step protocols are provided for preparing lysates of cells and yeast under a variety of conditions, for binding the antibody to a solid matrix, and for performing the actual immunoprecipitation. An additional method is provided for increasing the specificity of the technique by reprecipitating the antigen with the same or a different antibody. © 2016 by John Wiley & Sons, Inc.
- National Institutes of Health United States
- National Institute of Child Health and Human Development United States
- Child Health and Development Institute United States
Protein Denaturation, Magnetic Phenomena, Detergents, Saccharomyces cerevisiae, Antibodies, Microspheres, Solutions, Cell Adhesion, Animals, Humans, Immunoprecipitation, Glass
Protein Denaturation, Magnetic Phenomena, Detergents, Saccharomyces cerevisiae, Antibodies, Microspheres, Solutions, Cell Adhesion, Animals, Humans, Immunoprecipitation, Glass
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).23 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!