ABSTRACTMicrotubules (MTs) are essential cytoskeletal elements in all eukaryotes, playing critical roles in cell shape, intercellular organization, cell division, and cell motility. The organization of the MT network has undergone significant changes throughout plant evolution. Some MT structures, such as the preprophase band and phragmoplast, are innovations in plant lineages, while others, including the centriole and flagellum, have been lost over time. Bryophytes, consisting of mosses, liverworts, and hornworts, are the earliest land plants and occupy a key phylogenetic position in the evolution of MT organization. In the past two decades, advances in genomics, genetics, and cell imaging technologies have significantly enhanced our understanding of MT organization and function. Two representative species, Physcomitrium patens (moss) and Marchantia polymorph (liverwort), have become established model organisms, and new models for hornworts are emerging. In this review, we summarize the current knowledge of the MT cytoskeleton, drawing from early electron microscopy studies and recent advances in these emerging models. Our aim is to provide a comprehensive overview of the major MT array types and key factors involved in MT organization in bryophytes, offering insights into MT adaptation during plant evolution.