Quorum sensing (QS) allows bacteria to coordinate community‐wide behaviors such as biofilm formation, virulence, and symbiosis. The diazeniumdiolate valdiazen is identified in the opportunistic pathogen Burkholderia cenocepacia H111 as a novel quorum‐sensing signal, yet its protein interactome remains unexplored. In this study, a chemoproteomic pulldown approach is used to identify potential valdiazen‐binding proteins. For these pulldown experiments, a series of alkyne‐linked and biotin‐conjugated valdiazen probes are synthesized. Affinity‐based pulldown experiments using biotin‐valdiazen conjugates successfully identify several putative proteins including an ATP synthase subunit, a succinylglutamate desuccinylase/aspartoacylase, a granule‐associated protein, an acetyl‐CoA hydrolase, a serine protease and an OmpA/MotB precursor. Overall, this study provides insights into the valdiazen–protein interactome in Burkholderia cenocepacia H111, advancing our understanding of the role of valdiazen in bacterial QS.