Probing Ambiguous Base‐Pairs by Genetic Transformation with XNA Templates
Copyright policy )Probing Ambiguous Base‐Pairs by Genetic Transformation with XNA Templates
AbstractThe templating potential of anhydrohexitol oligonucleotides bearing ambiguous bases was studied in vivo, by using a selection screen for mosaic heteroduplex plasmids in Escherichia coli. 1,5‐Anhydro‐2,3‐dideoxy‐2‐(5‐nitroindazol‐1‐yl)‐D‐arabino‐hexitol showed the greatest ambiguity among the three nucleosides tested. At most two successive ambiguous bases could be tolerated on hexitol templates read in bacterial cells. Hexitol nucleosides bearing simplified heterocycles thus stand as promising monomers for generating random DNA sequences in vivo from defined synthetic oligonucleotides.
Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology, unnatural bases, XNA, Oligonucleotides, Chemistry, Medicinal, 0601 Biochemistry and Cell Biology, OLIGONUCLEOTIDES, Sugar Alcohols, Transformation, Genetic, Nucleic Acids, Pharmacology & Pharmacy, Base Pairing, IN-VIVO, 3404 Medicinal and biomolecular chemistry, NUCLEOSIDE, Science & Technology, 0304 Medicinal and Biomolecular Chemistry, Molecular Structure, Organic Chemistry, DNA, Templates, Genetic, [SDV] Life Sciences [q-bio], polymerization, universal bases, HNA, REPLICATION, ACID, POLYMERS, Life Sciences & Biomedicine, mutagenesis, HYBRIDIZATION
Biochemistry & Molecular Biology, 3101 Biochemistry and cell biology, unnatural bases, XNA, Oligonucleotides, Chemistry, Medicinal, 0601 Biochemistry and Cell Biology, OLIGONUCLEOTIDES, Sugar Alcohols, Transformation, Genetic, Nucleic Acids, Pharmacology & Pharmacy, Base Pairing, IN-VIVO, 3404 Medicinal and biomolecular chemistry, NUCLEOSIDE, Science & Technology, 0304 Medicinal and Biomolecular Chemistry, Molecular Structure, Organic Chemistry, DNA, Templates, Genetic, [SDV] Life Sciences [q-bio], polymerization, universal bases, HNA, REPLICATION, ACID, POLYMERS, Life Sciences & Biomedicine, mutagenesis, HYBRIDIZATION
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