Nonenzymatic Labeling of 5‐Hydroxymethylcytosine in Nanopore Sequencing

descriptionPublicationkeyboard_double_arrow_right Article 18 Jun 2013 English Publisher:WileyJournal:ChemBioChem, volume 14, pages 1,289-1,290 (issn: 1439-4227, eissn: 1439-7633,

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Authors: Xingyu, Lu; Chuan, He;

doi: 10.1002/cbic.201300342

pmid: 23780928

Nonenzymatic Labeling of 5‐Hydroxymethylcytosine in Nanopore Sequencing

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Abstract

Labeling chemically for a "single" result: A one-step, nonenzymatic 5hmC thiolation method mediated by bisulfite is highlighted. The 5hmC base can be chemically labeled in single-stranded DNA and detected by using nanopore sequencing.

Related Organizations

University of Chicago
United States

Keywords

Cytosine, Nanopores, 5-Methylcytosine, DNA, Single-Stranded, Sequence Analysis, DNA, DNA Methylation

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