AbstractWe have developed and characterized efficient caged compounds of the neurotransmitter octopamine. For derivatization, we introduced [6‐bromo‐8‐(diethylaminomethyl)‐7‐hydroxycoumarin‐4‐yl]methoxycarbonyl (DBHCMOC) and {6‐bromo‐7‐hydroxy‐8‐[(piperazin‐1‐yl)methyl]coumarin‐4‐yl}methoxycarbonyl (PBHCMOC) moieties as novel photo‐removable protecting groups. The caged compounds were functionally inactive when applied to heterologously expressed octopamine receptors (AmOctα1R). Upon irradiation with UV–visible or IR light, bioactive octopamine was released and evoked Ca2+ signals in AmOctα1R‐expressing cells. The pronounced water solubility of compounds 2–4 in particular holds great promise for these substances as excellent phototriggers of this important neurotransmitter.