Powered by OpenAIRE graph
Found an issue? Give us feedback
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao ChemBioChemarrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
ChemBioChem
Article . 2011 . Peer-reviewed
License: Wiley Online Library User Agreement
Data sources: Crossref
ChemBioChem
Article . 2012
Data sources: Europe PubMed Central
https://dx.doi.org/10.1002/cbi...
Article
Data sources: Microsoft Academic Graph
 View all 2 versions
Claim

Rational Design of Fluorescent Biosensor for Cyclic di‐GMP

descriptionPublicationkeyboard_double_arrow_right Article 21 Oct 2011 English Publisher:WileyJournal:ChemBioChem, volume 12, pages 2,753-2,758 (issn: 1439-4227, eissn: 1439-7633, Copyright policy )
Authors: Chun Loong, Ho; Siew Lee, Koh; Mary Lay Cheng, Chuah; Zhen, Luo; Wei Jian, Tan; Donovan Kian Soon, Low; Zhao-Xun, Liang;

doi: 10.1002/cbic.201100557

pmid: 22021215

Rational Design of Fluorescent Biosensor for Cyclic di‐GMP

Abstract

Messenger bagged: The design of a fluorophore-labeled protein biosensor for the bacterial messenger cyclic di-GMP is described. The biosensor responds to c-di-GMP with sub-micromolar sensitivity in a real-time fashion. The biosensor can be used for enzyme assays for diguanylate cyclases and c-di-GMP phosphodiesterases as well as the high-throughput screening of inhibitors.

Related Organizations
Keywords

Models, Molecular, Inhibitory Concentration 50, Spectrometry, Fluorescence, Biosensing Techniques, Cyclic GMP, Fluorescent Dyes

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 5
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Top 10%
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Average
Average
Top 10%
Upload OA version
Are you the author of this publication? Upload your Open Access version to Zenodo!
It’s fast and easy, just two clicks!
uploadUpload now