Research Progress and Prospects for Fructosyltransferases

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 22 Apr 2021 English Publisher:WileyJournal:ChemBioEng Reviews, volume 8, pages 200-212 (issn: 2196-9744, eissn: 2196-9744,

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Authors: Yitian Wang; Xiujie Shang; Fan Cao; Haiquan Yang;

doi: 10.1002/cben.202000011

Research Progress and Prospects for Fructosyltransferases

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Abstract

AbstractFructosyltransferases can be used to produce fructooligosaccharides from sucrose and are obtained from different sources, mostly microorganisms and plants. Heterologous expression and several mutagenesis methods were applied to improve the production of fructosyltransferases. The structures and characteristics of fructosyltransferases were investigated. Immobilization and protein engineering can improve their characteristics. In this review, screening and mutagenesis of microorganisms, heterologous expression, purification, characterization, structural analysis, immobilization, and protein engineering of fructosyltransferases are discussed. Future prospects in this field are also considered.

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