AbstractActinomycetes have being regarded as a treasure reservoir of various bioactive secondary metabolites and devoted many antibiotics in clinicals. Amycolatopsis sp. YNNP 00208 was isolated from a soil sample collected in Gaoligong Mountain area, Yunnan Province, China. Chemical investigation of its fermentation broth led to a new amide, baoshanmycin (1), and a new furanone derivative, 3‐(1,3‐dihydroxybutyl)‐4‐methylfuran‐2(H)‐one (2), together with eight known compounds, including two amides (3–4), four cyclic dipeptides (5–8), and two deoxyribonucleosides (9–10). Their structures were established on basis of the 1D‐ and 2D‐NMR spectroscopic data, along with the HR‐ESI‐MS experiments. Baoshanmycin (1) showed moderate antimicrobial activities against Candida albicans, and weak activities against Staphylococcus aureus, multi‐drug resistant Staphylococcus aureus, Bacillus subtilis, Listeria monocytogenes, fluconazole‐resistant Candida albicans. Baoshanmycin (1) presented strong antioxidant activity and moderate anti‐acetylcholinesterase activity. The other compound 3‐(1,3‐dihydroxybutyl)‐4‐methylfuran‐2(H)‐one (2) and the known compounds (3–10) showed moderate antioxidant activity.