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Rutin as Deoxyribonuclease I Inhibitor

descriptionPublicationkeyboard_double_arrow_right Article 10 Apr 2019 Slovenia Publisher:WileyJournal:Chemistry & Biodiversity, volume 16 (issn: 1612-1872, eissn: 1612-1880,

Copyright policy )Funded by:MESTD | Production of new dieteti..., MESTD | Plants and synthetic bioa..., MESTD | Obtaining, physicochemica...

Authors: Ana Kolarevic; Aleksandra Pavlovic; Aleksandra Djordjevic; Jelena Lazarevic; Sasa Savic; Gordana Kocic; Marko Anderluh; +1 Authors

doi: 10.1002/cbdv.201900069

pmid: 30883015

Rutin as Deoxyribonuclease I Inhibitor

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Abstract

AbstractDNase I inhibitory potential of water extract of nine Hypericum species (H. umbellatum, H. barbatum, H. rumeliacum, H. rochelii, H. perforatum, H. tetrapterum, H. olympicum, H. hirsutum, H. linarioides) and the most important Hypericum secondary metabolites (hypericin, hyperforin, quercetin, and rutin) was investigated. All examined Hypericum extracts inhibited DNase I with IC50 below 800 μg/ml, whereby H. perforatum was the most potent (IC50=391.26±68.40 μg/ml). Among the investigated Hypericum secondary metabolites, rutin inhibited bovine pancreatic DNase I in a non‐competitive manner with IC50 value of 108.90±9.73 μm. DNase I inhibitory ability of rutin was further confirmed on DNase I in rat liver homogenate (IC50=137.17±16.65 μm). Due to the involvement of DNase I in apoptotic processes the results of this study indicate the importance of frequent rutin and H. perforatum consumption in daily human nutrition. Rutin is a dietary component that can contribute to male infertility prevention by showing dual mechanism of sperm DNA protection, DNase I inhibition and antioxidant activity.

Country

Slovenia

Related Organizations

Institute of Information Science
Slovenia
University of Ljubljana
Slovenia
University of Niš
Serbia

Keywords

Flavonoids, Spectrometry, Mass, Electrospray Ionization, Binding Sites, Plant Extracts, Rutin, Farmacevtska kemija., hypericum, rutin, DNase I inhibition, molecular docking, apoptosis, male infertility, oxidative stress, DNA, Rats, Molecular Docking Simulation, Inhibitory Concentration 50, Liver, Catalytic Domain, Animals, Deoxyribonuclease I, info:eu-repo/classification/udc/615.4, Chromatography, High Pressure Liquid, Hypericum

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