Asperochramides A – D (1 – 4), a new natural product and three new indole diketopiperazine alkaloids, along with seven known analogs (5 – 11), were isolated from the ethyl acetate extract of Aspergillus ochraceus. Their structures were elucidated by extensive spectroscopic analyses, ECD calculation, and single‐crystal X‐ray diffraction analysis. Compounds 3 and 4 represent a rare group of indole diketopiperazine alkaloid with a 3‐hydroxyl‐2‐indolone moiety. The in vitro anti‐inflammatory effects of compounds 1 and 3 – 11 were investigated by using LPS‐stimulated murine macrophage RAW 264.7 cells. Compounds 1, 8, 10, and 11 showed potential anti‐inflammatory activities.