Development and validation of a sensitive LC–MS/MS assay for quantification of bruceine D in rat plasma

descriptionPublicationkeyboard_double_arrow_right Article 28 Apr 2016 English Publisher:WileyJournal:Biomedical Chromatography, volume 30, pages 1,873-1,876 (issn: 0269-3879, eissn: 1099-0801,

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Authors: Yining, Zhang; Yong, Zhen; Ying, Zhang; Shouyue, Liu;

doi: 10.1002/bmc.3724

pmid: 27012305

Development and validation of a sensitive LC–MS/MS assay for quantification of bruceine D in rat plasma

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Abstract

AbstractA sensitive LC–MS/MS method for the determination of bruceine D in rat plasma was developed. The analyte and IS were separated on a Luna C18 column (2.1 × 50 mm, 1.7 μm) using a mobile phase of acetonitrile and 0.1% formic acid in water (40:60, v/v) at a flow rate of 0.25 mL/min. The selected reaction monitoring mode was chosen to monitor the precursor‐to‐product ion transitions of m/z 409.2 → 373.2 for bruceine D and m/z 469.2 → 229.3 for IS using a negative ESI mode. The method was validated over a concentration range of 0.5–2000 ng/mL for bruceine D. Total chromatography time for each run was 3.5 min. The method was successfully applied to a pharmacokinetic study of bruceine D in rats. Copyright © 2016 John Wiley & Sons, Ltd.

Related Organizations

Northern Jiangsu People's Hospital
China (People's Republic of)
Jilin University
China (People's Republic of)
First Hospital of Jilin University
China (People's Republic of)
Jilin University
China (People's Republic of)

Keywords

Male, Spectrometry, Mass, Electrospray Ionization, Quassins, Reproducibility of Results, Antineoplastic Agents, Phytogenic, Rats, Rats, Sprague-Dawley, Antimalarials, Limit of Detection, Tandem Mass Spectrometry, Brucea, Animals, Chromatography, High Pressure Liquid

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