Awakening dormant glycosyltransferases in CHO cells with CRISPRa
Copyright policy )Awakening dormant glycosyltransferases in CHO cells with CRISPRa
AbstractChinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced. This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.
- University of California United States
- University of California, San Diego United States
- University of California
- KAIST Korea (Republic of)
- UNIVERSITY OF CALIFORNIA United States
Gene Editing, St6gal11, Glycosylation, glycosylation, CHO, Mgat3, Glycosyltransferases, CHO Cells, Biological Sciences, CRISPRa, Cricetulus, Phenotype, Polysaccharides, Cricetinae, Genetics, Animals, St6gal1, Biochemistry and Cell Biology, CRISPR-Cas Systems, Biotechnology
Gene Editing, St6gal11, Glycosylation, glycosylation, CHO, Mgat3, Glycosyltransferases, CHO Cells, Biological Sciences, CRISPRa, Cricetulus, Phenotype, Polysaccharides, Cricetinae, Genetics, Animals, St6gal1, Biochemistry and Cell Biology, CRISPR-Cas Systems, Biotechnology
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