Serum‐free media in hybridoma culture and monoclonal antibody production

descriptionPublicationkeyboard_double_arrow_right Article 05 Oct 1988 English Publisher:WileyJournal:Biotechnology and Bioengineering, volume 32, pages 1,015-1,028 (issn: 0006-3592, eissn: 1097-0290,

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Authors: M C, Glassy; J P, Tharakan; P C, Chau;

doi: 10.1002/bit.260320809

pmid: 18587819

Serum‐free media in hybridoma culture and monoclonal antibody production

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Abstract

AbstractThe replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum‐free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum‐free formulations and selection rules for the serum‐free ingredients. In general, serum‐free media which are “lipid rich” can sustain cell growth rates approaching that of serum supplemented cultures. Specific antibody secretion rate, however, is usually higher in serum‐free media, irrespective of the lipid content.

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