Preparation and properties of immobilized amyloglucosidase

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1981 English Publisher:WileyJournal:Biotechnology and Bioengineering, volume 23, pages 2,273-2,282 (issn: 0006-3592, eissn: 1097-0290,

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Authors: V. S. Nithianandam; K. S. V. Srinivasan; K. Thomas Joseph; M. Santappa;

doi: 10.1002/bit.260231010

Preparation and properties of immobilized amyloglucosidase

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Abstract

AbstractAmyloglucosidase was immobilized on a copolymer of methyl methacrylate and 2‐dimethylaminoethyl methacrylate. The resulting immobilized amyloglucosidase has 19% of the soluble enzyme specific activity. The pH optimum of immobilized amyloglucosidase is shifted towards acidity by 1.9 units. The temperature optimum of immobilized enzyme is shifted upward by 5°C. The immobilized amyloglucosidase has the maximum stability at pH 4.6, whereas the soluble enzyme has maximum stability at pH 5.5. While soluble amyloglucosidase has a maximum thermal stability at 50°C, the stability of the immobilized amyloglucosidase steadily decreases with the increase in temperature.

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Central Leather Research Institute
India

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