Enzymatic biosynthesis of cephalexin

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1980 English Publisher:WileyJournal:Biotechnology and Bioengineering, volume 22, pages 1,237-1,247 (issn: 0006-3592, eissn: 1097-0290,

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Authors: D K, Rhee; S B, Lee; J S, Rhee; D D, Ryu; J, Hospodka;

doi: 10.1002/bit.260220610

pmid: 7378557

Enzymatic biosynthesis of cephalexin

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Abstract

AbstractThe reaction kinetics of the enzymatic of cephalexin from 7‐aminodea‐cetoxy cephalosporanic acid and phenylglycine methylester was studied using the synthesizing enzyme obtained from Xanthomonas citri. The activation energy, Km value for 7‐aminodeacetoxy cephalosporanic acid and phenylglycine methylester, and Ki value for phenylglycine methylester were determined as 8.63 kcal/mol, 3.7mM, 14.5mM, and 70mM, respectively. The enzyme was found to be constitutive and susceptible to deactivation.

Related Organizations

Korean Association Of Science and Technology Studies
Korea (Republic of)

Keywords

Oxygen, Cephalexin, Kinetics, Xanthomonas, Enzyme Induction, Penicillin Amidase, Benzoates, Amidohydrolases, Phenylacetates

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