Immobilization of microbial cells containing NAD‐kinase

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1979 English Publisher:WileyJournal:Biotechnology and Bioengineering, volume 21, pages 1,019-1,030 (issn: 0006-3592, eissn: 1097-0290,

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Authors: T, Hayashi; Y, Tanaka; K, Kawashima;

doi: 10.1002/bit.260210607

pmid: 35256

Immobilization of microbial cells containing NAD‐kinase

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Abstract

AbstractMicrobial cells having NAD‐kinase activity, Brevibacterium ammoniagenes, were immobilized by the radiation‐copolymerization method under low temperature with the activity recovery of more than 80%. Compared to the native microbial cells the immobilized cells were more stable against heat and pH change. The immobilized cells were subjected to the 5 hr reaction repeatedly 20 times without any activity loss.

Related Organizations

Food Research Institute
Japan
Ministry of Agriculture, Forestry and Fisheries
Japan

Keywords

Kinetics, Gamma Rays, Polymers, Phosphotransferases, Temperature, Brevibacterium, Hydrogen-Ion Concentration, Enzymes, Immobilized, NAD

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