Large‐scale purification of Qβ‐virus by differential centrifugation

descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1974 English Publisher:WileyJournal:Biotechnology and Bioengineering, volume 16, pages 847-851 (issn: 0006-3592, eissn: 1097-0290,

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Authors: W C, McGregor; J J, Round; A H, Ramel;

doi: 10.1002/bit.260160611

pmid: 4600533

Large‐scale purification of Qβ‐virus by differential centrifugation

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Abstract

AbstractBacterial RNA virus, Qβ, has been purified in gram amounts by differential centrifugation. Final separation of the virus from host E. coli rRNA was based on the density differential in the pellet. The method provided a simplified alternative to the more conventional rate zonal or isopycnic zonal centrifugation techniques.

Related Organizations

Roche (Switzerland)
Switzerland

Keywords

RNA, Ribosomal, Centrifugation, Density Gradient, Escherichia coli, RNA Viruses, RNA, Viral, Hydrogen-Ion Concentration, Coliphages, Dialysis

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