Detection of chrysotile asbestos by using a chrysotile‐binding protein
Copyright policy )Detection of chrysotile asbestos by using a chrysotile‐binding protein
AbstractIn the current studies, we found that the DksA protein from Escherichia coli binds strongly to chrysotile, which is the most commonly used form of asbestos. We developed a convenient colorimetric assay for chrysotile using a fusion of DksA and alkaline phosphatase along with 5‐bromo‐4‐chloro‐3‐indolyl‐phosphate and nitro blue tetrazolium as substrates. Also, using a fusion of DksA and green‐fluorescent protein, we were able to detect chrysotile by fluorescence microscopy. Biotechnol. Bioeng. 2008;99: 285–289. © 2007 Wiley Periodicals, Inc.
- Hiroshima University Japan
Asbestos, Serpentine, Microscopy, Fluorescence, Escherichia coli Proteins, Green Fluorescent Proteins, Colorimetry, Alkaline Phosphatase, Recombinant Proteins
Asbestos, Serpentine, Microscopy, Fluorescence, Escherichia coli Proteins, Green Fluorescent Proteins, Colorimetry, Alkaline Phosphatase, Recombinant Proteins
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