AbstractThe Ile‐Lys‐Val‐Ala‐Val (IKVAV) containing peptide, A208 (AASIKVAVSADR, mouse laminin α1 chain 2097–2108), was recently found to form amyloid‐like fibrils. Fibril formation is critical for its biological activities, including promotion of cell adhesion and neurite outgrowth. In the present study, we designed multifunctional peptide fibrils using the A208 peptide and an Arg‐Gly‐Asp (RGD)‐containing fibronectin active sequence for biomedical applications. The fibronectin active sequence GRGDS (FN) or a scrambled sequence RSGGD (SC) were conjugated to either A208 or to A208S (AASVVIAKSADR), a scrambled peptide of A208, with a glycine as a spacer. The FN‐A208 and SC‐A208 peptides formed a gel and were stained with Congo red similar to that of A208, but FN‐A208S and SC‐A208S did not form a gel. These results indicate that FN‐A208 and SC‐A208 form amyloid‐like fibrils similar to A208. A208 and SC‐A208 promoted cell attachment with filopodia formation, and this adhesion was inhibited by the IKVAV‐containing peptide, but not by EDTA or a GRGDS peptide. FN‐A208 promoted cell attachment with well‐organized actin stress fibers, and this adhesion was partially inhibited by either EDTA, GRGDS, or IKVAV. These data suggest that A208 binds to only IKVAV receptor(s) while the FN‐A208 interacts with both integrins and the IKVAV receptor(s). We conclude that multifunctional peptide fibrils can be designed by conjugation of active peptides on A208 and that this construct has potential to serve as a bioadhesive for tissue regeneration and engineering. © 2004 Wiley Periodicals, Inc. Biopolymers (Pept Sci), 2004