Bovine serum albumin interacts with bacterial luciferase
Copyright policy )Bovine serum albumin interacts with bacterial luciferase
AbstractBovine serum albumin (BSA) affects the amount of light obtained from bacterial luciferase by competing with luciferase for one of the luciferase substrates, the aldehyde. At low aldehyde concentrations BSA behaves as an inhibitor, but at high aldehyde concentrations BSA relieves substrate inhibition. BSA reversibly binds decanal with a Ksi = 3.36 μmol/l, approximately half the affinity of luciferase for decanal (KM = 1.5 μmol/l). BSA also increased the rate of intermediate II dark decay. The data suggest that this involves a direct protein‐protein (BSA‐luciferase) interaction.
- Harvard University United States
- Florida International University United States
Aldehydes, Kinetics, Luminescent Measurements, Animals, Cattle, Serum Albumin, Bovine, In Vitro Techniques, Reference Standards, Luciferases, Vibrio
Aldehydes, Kinetics, Luminescent Measurements, Animals, Cattle, Serum Albumin, Bovine, In Vitro Techniques, Reference Standards, Luciferases, Vibrio
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).10 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!