AbstractAsymmetric cell division generates two cells that contain different regulatory proteins and express different fates. In an example of asymmetric cell division from B. subtilis, a site on the membrane of the dividing cell is chosen to establish the initial asymmetry. Recent results(1,2) show that a key regulatory protein, SpollE, is localized to one side of a sporulating B. subtilis cell, and subsequently functions in an asymmetric manner. SpollE is a phosphatase at the beginning of a regulatory cascade that leads to activation of a cell fate‐determining transcription factor in only one daughter cell.