DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis
Copyright policy )DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis
AbstractDNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to differentiate between these two conjugation patterns. Results showed that DNAzyme–aptamer has more catalytic activity and efficiency than aptamer–DNAzyme. Thereby, DNAzyme–aptamer with its superior efficiency can be used for design and development of more sensitive aflatoxin B1 DNA based biosensors.
- Shahid Beheshti University of Medical Sciences Iran (Islamic Republic of)
- Tarbiat Modares University Iran (Islamic Republic of)
- Ahvaz Jundishapur University of Medical Sciences Iran (Islamic Republic of)
Aflatoxin B1, Benzidines, Biosensing Techniques, DNA, Catalytic, Hydrogen Peroxide, Aptamers, Nucleotide, Chromogenic Compounds, Oxidation-Reduction, Peroxidase
Aflatoxin B1, Benzidines, Biosensing Techniques, DNA, Catalytic, Hydrogen Peroxide, Aptamers, Nucleotide, Chromogenic Compounds, Oxidation-Reduction, Peroxidase
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