AbstractThe subfamily Bombycinae Latreille, [1802] is an important silk‐producing group, including well‐known economical insects. Although there are many studies on the development of these economic insects, the relationships between genera/species of this subfamily are still unclear. Two data sets of mitochondrial genomes, 13 protein‐coding genes (13PCGs) and 13PCGs‐AA, were used to estimate phylogenetic relationships based on the maximum likelihood and Bayesian inference methods. The results strongly support the subfamily Bombycinae as a monophyletic group divided into two clades.