ABSTRACTNatural compounds, such as pequi oil, could be added to collagen/gelatin mixtures to improve its properties. Gels characterized by rheological analysis indicated modifications in entanglement network of the mixtures after pequi oil addition. Films processed by casting were analyzed by mechanical and thermal properties, contact angle, and scanning electron microscopy (SEM). The pequi oil addition reduced the contact angle values due to the addition of carboxyl groups found in the palmitic and oleic acid chains of pequi oil. The rough characteristic observed by SEM images was a result of a good homogenization process after oil incorporation. In addition, pequi oil addition decreased the tensile forces and elongation of the films and increased the thermal stability possibly due to lower inter‐ and intra‐molecules interactions. The results suggested a good compatibility between pequi oil and biopolymers and the potential application of this natural compound to increase the wound healing process. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 46627.