AbstractThe endodermal layer of the human yolk sac was examined three‐dimensionally with light microscopy on serial sections using scanning electron microscopy and transmission electron microscopy to find the origin of hemo poiesis in the yolk sac. Cell‐labelling techniques were also employed using the monoclonal anti‐transferrin receptor antibody. Orifices of the endodermal and intracellular tubules facing the yolk‐sac cavity were demonstrated on the endodermal surface. Various‐sized blood cells in various stages of differentiation and maturation were distributed in the yolk‐sac cavity and tubules and were observed also at the orifices of the tubules. The morphological and the immunological findings suggest that blood cells with large nuclei in the endodermal layer are the most immature. The present results suggest that blood cells originate from the endodermal layer and are carried to the embryo through the yolk sac cavity and the vitel‐line duct. It is probable that the endodermal and intracellular systems of tubules have an important role in the transport of blood cells, including stem cells.