Murine Norovirus: Propagation, Quantification, and Genetic Manipulation
Copyright policy )Murine Norovirus: Propagation, Quantification, and Genetic Manipulation
AbstractMurine norovirus (MNV) is a positive‐sense, plus‐stranded RNA virus in theCaliciviridaefamily. It is the most common pathogen in biomedical research colonies. MNV is also related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Like the human noroviruses, MNV is an enteric virus that replicates in the intestine and is transmitted by the fecal‐oral route. MNV replicates in murine macrophages and dendritic cells in cells in culture and in the murine host. This virus is often used to study mechanisms in norovirus biology, because human noroviruses are refractory to growth in cell culture. MNV combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Herein, we describe a panel of techniques that are commonly used to study MNV biology.Curr. Protoc. Microbiol33:15K.2.1‐15K.2.61. © 2014 by John Wiley & Sons, Inc.
- University of Michigan–Flint United States
- University of Michigan Medical School United States
- University Of Cambridge
- University of Cambridge United Kingdom
- University of Cambridge
Virus Cultivation, purification, Cesium, Enzyme-Linked Immunosorbent Assay, Genome, Viral, Virus Replication, Polymerase Chain Reaction, Cell Line, reverse genetics, Mice, Chlorides, Health Sciences, Centrifugation, Density Gradient, Animals, Humans, murine norovirus, Recombination, Genetic, Norovirus, quantification, Reverse Genetics, transfection, Microbiology and Immunology, Mutagenesis
Virus Cultivation, purification, Cesium, Enzyme-Linked Immunosorbent Assay, Genome, Viral, Virus Replication, Polymerase Chain Reaction, Cell Line, reverse genetics, Mice, Chlorides, Health Sciences, Centrifugation, Density Gradient, Animals, Humans, murine norovirus, Recombination, Genetic, Norovirus, quantification, Reverse Genetics, transfection, Microbiology and Immunology, Mutagenesis
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