BACKGROUND. The Discovery of Green Fluorescent Protein. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF GREEN FLUORESCENT PROTEIN. Biochemical and Physical Properties of Green Fluorescent Protein. The Three--Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design. Molecular Biology and Mutation of Green Fluorescent Protein. APPLICATIONS OF GREEN FLUORESCENT PROTEIN. The Uses of Green Fluorescent Protein in Caenorhabditis elegans. The Uses of Green Fluorescent Protein in Prokaryotes. The Uses of Green Fluorescent Protein in Yeasts. The Uses of Green Fluorescent Protein in Drosophila. The Uses of Green Fluorescent Protein in Plants. The Uses of Green Fluorescent Protein in Trangenic Vertebrates. The Uses of Green Fluorescent Protein in Mammalian Cells. METHODS AND PROTOCOLS. Expressioin of Green Fluorescent Protein. Specimen Preparation. Visualization of Green Fluorescent Protein. Documenting Green Fluorescent Protein. Appendix. Index.