Transfection by Electroporation
Copyright policy )Transfection by Electroporation
AbstractElectroporation–the use of high‐voltage electric shocks to introduce DNA into cells–can be used with most cell types, yields a high frequency of both stable transformation and transient gene expression, and, because it requires fewer steps, can be easier than alternate techniques. In this unit, the basic protocol describes the electroporation of mammalian cells and an alternate protocol outlines modifications for preparation and transfection of plant protoplasts.
- University of Colorado Anschutz Medical Campus United States
- University of South Florida United States
- Old Dominion University United States
- Harvard University United States
Mammals, Protoplasts, Cell Membrane, Gene Expression, Reproducibility of Results, DNA, Plants, Transfection, Cell Line, Electroporation, Plant Cells, Humans, Animals, Indicators and Reagents, Cells, Cultured
Mammals, Protoplasts, Cell Membrane, Gene Expression, Reproducibility of Results, DNA, Plants, Transfection, Cell Line, Electroporation, Plant Cells, Humans, Animals, Indicators and Reagents, Cells, Cultured
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