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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Current Protocols in...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Current Protocols in Cell Biology
Article . 1999 . Peer-reviewed
License: Wiley Online Library User Agreement
Data sources: Crossref
Current Protocols in Cell Biology
Article . 2008
Data sources: Europe PubMed Central
https://dx.doi.org/10.1002/047...
Article
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In Vitro Transcription

descriptionPublicationkeyboard_double_arrow_right Article 01 Apr 1999 English Publisher:WileyJournal:Current Protocols in Cell Biology, volume 2 (issn: 1934-2500, eissn: 1934-2616, Copyright policy )
Authors: R T, Kamakaka; W L, Kraus;

doi: 10.1002/0471143030.cb1106s02

pmid: 18228311

In Vitro Transcription

Abstract

AbstractIn vitro analysis of transcription and the factors that play a role in transcription require preparation of an extract that faithfully reproduces in vivo transcription. This unit describes protocols for generating extracts of mammalian cells and Drosophila embryos that are competent for in vitro transcription reactions using naked DNA or chromatin templates and for primer extension analysis of transcripts.

Related Organizations
Keywords

Cell Extracts, Transcription, Genetic, Animals, Humans, Drosophila, In Vitro Techniques, Molecular Biology, HeLa Cells

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
2
Average
Average
Average
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