
AbstractImmunohistochemistry is a vastly diverse and essential method for localization of proteins in cells and tissues. This unit presents methods for labeling proteins in suspension and adherent cultures and in tissue sections, using detection methods for both fluorescence and bright‐field microscopy. Choices of antibodies and detection methods are discussed, and detailed troubleshooting guidelines are provided. Curr. Protocol. Cytom. 48:12.16.1‐12.16.10. © 2009 by John Wiley & Sons, Inc.
Tissue Fixation, Animals, Biotin, Fluorescent Antibody Technique, Humans, Indicators and Reagents, Streptavidin, Immunohistochemistry, Antibodies
Tissue Fixation, Animals, Biotin, Fluorescent Antibody Technique, Humans, Indicators and Reagents, Streptavidin, Immunohistochemistry, Antibodies
| citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | 9 | |
| popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network. | Top 10% | |
| influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically). | Top 10% | |
| impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network. | Average |
