Assays for the Mannan‐Binding Lectin Pathway
Copyright policy )Assays for the Mannan‐Binding Lectin Pathway
AbstractThis unit contains protocols that can be used to measure mannan‐binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti‐MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.
- Aarhus University Denmark
Mannose-Binding Protein-Associated Serine Proteases, Humans, Complement Pathway, Mannose-Binding Lectin, Enzyme-Linked Immunosorbent Assay, Mannose-Binding Lectin
Mannose-Binding Protein-Associated Serine Proteases, Humans, Complement Pathway, Mannose-Binding Lectin, Enzyme-Linked Immunosorbent Assay, Mannose-Binding Lectin
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).11 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!