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Production of Monoclonal Antibodies

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1992 English Publisher:WileyJournal:Current Protocols in Immunology, volume 102 (issn: 1934-3671, eissn: 1934-368X,

Authors: Wayne M, Yokoyama; Michelle, Christensen; Gary Dos, Santos; Diane, Miller; Jason, Ho; Tao, Wu; Michael, Dziegelewski; +1 Authors

doi: 10.1002/0471142735.im0205s102 , 10.1002/0471142956.cya03js37 , 10.1002/0471142735.im0205s03 , 10.1002/0471143030.cb1601s03

pmid: 18228333 , 24510488 , 18770829

Production of Monoclonal Antibodies

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Abstract

AbstractThis unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one‐step selection and cloning of hybridomas utilizing a semi‐solid methylcellulose‐based medium (ClonaCell‐HY from StemCell Technologies). Curr. Protoc. Immunol. 102:2.5.1‐2.5.29. © 2013 by John Wiley & Sons, Inc.

Related Organizations

University of California System
United States
Washington State University
United States
University of California, San Francisco
United States
Stem Cell Technology (Taiwan)
Taiwan
University of Mary
United States

Keywords

B-Lymphocytes, Hybridomas, Recombinant Fusion Proteins, Cell Culture Techniques, Antibodies, Monoclonal, Enzyme-Linked Immunosorbent Assay, Methylcellulose, Cell Line, Clone Cells, Cell Fusion, Epitopes, Antibody Specificity, Culture Media, Conditioned, Immunoglobulin G, Immunologic Techniques, Animals, Humans, Immunization

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