descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1997 English Publisher:WileyJournal:Current Protocols in Molecular Biology, volume 37 (issn: 1934-3639, eissn: 1934-3647,

Authors: Steven A. Fuller; Miyoko Takahashi; John G.R. Hurrell;

doi: 10.1002/0471142727.mb1111s37

pmid: 18265069

Purification of Monoclonal Antibodies

- Summary
- Subjects
- Metrics

Abstract

AbstractThe uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A‐Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion‐exchange chromatography for the preparation of contaminant‐free antibody.

Keywords

Immunologic Techniques, Antibodies, Monoclonal, Ascites, Chromatography, Affinity

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	5
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average