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image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Current Protocols in...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Current Protocols in Molecular Biology
Article . 1988 . Peer-reviewed
License: Wiley Online Library User Agreement
Data sources: Crossref
Current Protocols in Molecular Biology
Article . 2008
Data sources: Europe PubMed Central
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cDNA Libraries

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 1988 English Publisher:WileyJournal:Current Protocols in Molecular Biology, volume 1 (issn: 1934-3639, eissn: 1934-3647, Copyright policy )
Authors: D D, Moore;

doi: 10.1002/0471142727.mb0502s01

pmid: 18265246

Abstract

AbstractThis unit provides a basic description of issues that require careful consideration before undertaking construction of cDNA libraries. Topics include: generating a double‐stranded DNA copy of the mRNA, the quality of the mRNA, the relative abundance of the clone of interest, the choice of screening method, the size of the library that will be necessary to include the clone of interest, and choices of linkers or adaptors for insertion into the vector.

Keywords

DNA, Complementary, RNA, Messenger, Cloning, Molecular, Bacteriophage lambda

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
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