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Techniques for Mammalian Cell Tissue Culture

Name: Techniques for Mammalian Cell Tissue Culture
Creator: Mary C. Phelan
Keywords: Cryopreservation, Mammals, 0301 basic medicine, 0303 health sciences, Antifungal Agents, Hemocytes, Cell Culture Techniques, Sterilization, Cell Count, Toxicology

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1994 English Publisher:WileyJournal:Current Protocols in Human Genetics, volume 51 (issn: 1934-8266, eissn: 1934-8258,

Authors: Mary C. Phelan;

doi: 10.1002/0471142727.hga03gs51 , 10.1002/0471140864.psa03cs46 , 10.1002/0471142727.nsa03bs38 , 10.1002/0471142905.hga03gs02 , 10.1002/0471140856.txa03bs02 , 10.1002/0471140856.txa03bs33 , 10.1002/0471140864.psa03cs07 , 10.1002/0471142727.mba03fs35 , 10.1002/0471142956.cya03bs36 , 10.1002/0471142727.mba03fs74 , 10.1002/0471142956.cya03bs00 , 10.1002/0471142301.nsa03bs02

pmid: 18770828 , 18428653 , 18428384 , 18265370 , 18429293 , 20972966

Techniques for Mammalian Cell Tissue Culture

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Abstract

AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.

Related Organizations

Children’s Hospital at Erlanger
United States
Greenwood Genetic Center
United States
Molecular Pathology Laboratory Network (United States)
United States

Keywords

Cryopreservation, Mammals, Antifungal Agents, Hemocytes, Cell Culture Techniques, Sterilization, Cell Count, Toxicology, Anti-Bacterial Agents, Cell Line, Culture Media, Tissue Culture Techniques, Infertility, Freezing, Humans, Animals, Indicators and Reagents, Trypsin, Cells, Cultured

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	119
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Top 10%
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Top 1%
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average