Powered by OpenAIRE graph
Found an issue? Give us feedback
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Current Protocols in...arrow_drop_down
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Current Protocols in Neuroscience
Article . 1997 . Peer-reviewed
License: Wiley Online Library User Agreement
Data sources: Crossref
image/svg+xml Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao Closed Access logo, derived from PLoS Open Access logo. This version with transparent background. http://commons.wikimedia.org/wiki/File:Closed_Access_logo_transparent.svg Jakob Voss, based on art designer at PLoS, modified by Wikipedia users Nina and Beao
Current Protocols in Cell Biology
Article . 2003 . Peer-reviewed
License: Wiley Online Library User Agreement
Data sources: Crossref
Current Protocols in Cell Biology
Article . 2008
Data sources: Europe PubMed Central
https://dx.doi.org/10.1002/047...
Article
Data sources: Microsoft Academic Graph
https://dx.doi.org/10.1002/047...
Article
Data sources: Microsoft Academic Graph
 View all 3 versions
Claim

This Research product is the result of merged Research products in OpenAIRE.

You have already added 0 works in your ORCID record related to the merged Research product.

Optimization of Transfection

descriptionPublicationkeyboard_double_arrow_right Article 01 Nov 1997 English Publisher:WileyJournal:Current Protocols in Neuroscience, volume 1 (issn: 1934-8584, eissn: 1934-8576, Copyright policy )
Authors: John K, Rose;

doi: 10.1002/0471142301.nsa01bs01 , 10.1002/0471143030.cb2007s19

pmid: 18228431

Optimization of Transfection

Abstract

AbstractThe single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate‐mediated gene transfer, DEAE‐dextran‐mediated gene transfer, electroporation, and liposome‐mediated transfection. Fusion techniques such as protoplast fusion and microinjection may also be considered.

Related Organizations
Keywords

Calcium Phosphates, BALB 3T3 Cells, Time Factors, DEAE-Dextran, DNA, Transfection, Cell Line, Mice, Electroporation, Liposomes, Animals, Humans, HeLa Cells

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 5
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Average
Average
Average
Upload OA version
Are you the author of this publication? Upload your Open Access version to Zenodo!
It’s fast and easy, just two clicks!
uploadUpload now