Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F‐actin
Copyright policy )Fluorescence Recovery After Photobleaching (FRAP) with a Focus on F‐actin
AbstractIn this unit on fluorescence recovery after photobleaching (FRAP), an imaging approach to study protein‐protein interactions in situ is described. The protocols presented use confocal microscopy to examine the mobility of a fluorescent protein by measuring the recovery of the protein in a bleached area. The data gained in FRAP studies is qualitative and yields insight into relative binding affinity, binding characteristics, and the effect of treatments or mutations on protein mobility. Curr. Protoc. Neurosci. 61:2.17.1‐2.17.12. © 2012 by John Wiley & Sons, Inc.
- Philadelphia College of Osteopathic Medicine United States
Microscopy, Confocal, Time Factors, actins, GFPactin cytoskeleton, Green Fluorescent Proteins, fluorescence recovery after photobleaching, Neurosciences, time factors, Actins, Actin Cytoskeleton, confocal, Neurology, FRAP, microscopy, Medicine and Health Sciences, cells, cultured, green fluorescent proteins, actin, protein mobility, Cells, Cultured, Fluorescence Recovery After Photobleaching
Microscopy, Confocal, Time Factors, actins, GFPactin cytoskeleton, Green Fluorescent Proteins, fluorescence recovery after photobleaching, Neurosciences, time factors, Actins, Actin Cytoskeleton, confocal, Neurology, FRAP, microscopy, Medicine and Health Sciences, cells, cultured, green fluorescent proteins, actin, protein mobility, Cells, Cultured, Fluorescence Recovery After Photobleaching
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).13 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!