Measurement of Ferrochelatase Activity
Copyright policy )Measurement of Ferrochelatase Activity
AbstractFerrochelatase is the terminal enzyme in the heme biosynthesis pathway. Under anaerobic conditions it catalyzes the insertion of ferrous iron into the protoporphyrin IX ring to form protoheme. In the absence of iron and under aerobic conditions, the enzyme will use zinc or mercury as a substitute. The assay described in this unit uses zinc under aerobic conditions and the artificial substrate mesoporphyrin to analyze the formation of zinc‐mesoporphyrin by HPLC with UV or fluorescence detection.
Spectrometry, Fluorescence, Mesoporphyrins, Metalloporphyrins, Spectrophotometry, Animals, Humans, Mitochondria, Liver, Chromatography, High Pressure Liquid, Ferrochelatase, Enzyme Assays
Spectrometry, Fluorescence, Mesoporphyrins, Metalloporphyrins, Spectrophotometry, Animals, Humans, Mitochondria, Liver, Chromatography, High Pressure Liquid, Ferrochelatase, Enzyme Assays
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