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The Quantitative Determination of Etonium by the Enzymatic Kinetic-Spectrophotometric Method

The Quantitative Determination of Etonium by the Enzymatic Kinetic-Spectrophotometric Method

Abstract

The use of the cholinesterase enzyme as a component of the analytical system has made it possible to develop a new kinetic-spectrophotometric method, which is alternative to the pharmacopoeial method, for determining the quaternary ammonium salt – ethonium in the substance and a dosage form. This method is characterized by high sensitivity and specificity, and is relatively cheap. The relative standard deviation of the procedure does not exceed 2.7 %, and the limit of quantification is 17 ng mL–1.

Використання ферменту холінестерази як компонента аналітичної системи дозволило розробити нову, альтернативну фармакопейній, кінетико-спектрофотометричну методику визначення четвертинної амонійної солі етонію в субстанції та лікарському засобі. Методика характеризується високою чутливістю і специфічністю та є відносно дешевою. Відносне стандартне відхилення методики не перевищувало 2,7 %, а межа визначення становила 17 нг мл–1.

Keywords

етоній, ethonium, поверхнево-активні речовини, cholinesterase, холінестераза, surfactant, ацетилхолін, acetylcholine

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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