Various schemes of the application of sterilizing agents of systemic action (antibiotics, fungicides) in the complex with sodium hypochlorite and various types of Agapanthus umbellatus explants are tested. The effect of primary explants origin on the formation of plant-regenerants is established. Among the plants of Charlotte sort, which were grown for a long time in the native conditions (in situ), bloomed and reproduced vegetatively, were received the explants with the higher rate of decontamination compared with the plants, which were grown from the seeds and formed buds for the first time. The place of their isolation from the donor plant in vivo also has had influence on the decontamination effectiveness of Charlotte sort. The lowest effectiveness was observed under using axillary buds which were isotated from the underground rhizomes. Using foundations inflorescences as explants during the introduction of sort Charlotte Agapanthus to culture in vitro gave an opportunity to get 53% of alive explants and decontamination effectiveness was 15%. This method was improved by us later following the results of extra decontamination effectiveness test, which were used with a mixture of sodium hypochlorite and potassium permanganate. The effectiveness of using Fundazol fungicide (benomil) as an extra decontaminant statistically didn’t differ from the control. High efficiency of the application of Previcur Energy 840 SL and Maxim Forte 050 FS preparation as an additional decontaminant according to all the examined parameters (survival rate of explants, index of decontamination, outcrop of regenerants from explants) is detected. Maxim Forte 050 FS prevailed the control in all indications, but it conceded to Previcur Energy 840 SL. Using donors explants with the age of 30 and 45 days predetermined low survival rate (27–58%). Appropriateness of the application of plants-regenerants aged 90 days for the clonal micropropagation of agapanthus of Charlotte, Вlack magic varieties is tested.

Випробувано різні схеми застосування стерилізаційних агентів системної дії (антибіотики, фунгіциди) у комплексі з гіпохлоритом натрію та різні види експлантів. Встановлено вплив походження первинних експлантів на утворення рослин-регенерантів. Виявлено високу ефективність застосування як додаткової деконтамінації препаратів Превікур Енерджі 840 SL та Максим Форте 050 FS за усіма досліджуваними показниками: часткою виживання експлантів, показником деконтамінації, виходом регенерантів з експлантів. Обґрунтовано доцільність використання рослин-донорів експлантів віком 90 днів для клонального мікророзмноження агапантусу сортів Шарлота, Чорна магія.