Yaws, a prevalent tropical disease primarily affecting children, is characterized by the presence of erythematous lesions predominantly found in the lower limb. Through a comprehensive multi-locus analysis of 255 fully typed clincal isolates, it was discovered that three allelic profiles (APs) namely J11, S22 and T13 are present. In this study, we present a unique case of a five-year-old male patient from Papaua New Guinea (PNG) who was found to be coinfected with two TPE strains, exhibiting allelic profiles J11 and T13. While the presence of both J11 and T13 genotypes was originally detected by double peaks present in the Sanger sequencing reads, cloning of PCR products and the subsequent sequencing of individual clones proved that there is a combination of two different genotypes in the original sample. Interestingly, about 21% of analyzed clones showed chimeric character, combined sequences of both J11 and T13 genotypes in a single sequence.